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阿拉丁®内参抗体

2025-01-15

阿拉丁®内参抗体


引言

          内参抗体是指用于标准化和量化蛋白质表达水平的抗体,它们通常针对在细胞中表达水平相对恒定的“管家基因”(Housekeeping Genes)编码的蛋白质。管家基因在不同细胞类型、组织和生理状态下表达相对稳定,因此常作为实验中的一种参照标准,以确保实验结果的准确性和可比性。

 

         选择合适的内参蛋白时,除了考虑靶标蛋白和内参蛋白在样本中的表达量外,还需要考虑内参蛋白分子量、靶标蛋白分子量、亚细胞分布、以及实验所涉及的处理等。

 

         常见的内参蛋白、蛋白大小及其位置分布如下:



        注:内参蛋白分子量主要依据Uniprot官网(https://www.uniprot.org/)预测所得,仅供参考。

 

        根据内参蛋白在体内的分布,将内参抗体产品分为位于细胞骨架、细胞核、细胞膜、细胞质/全细胞、线粒体和血清六类。


位于细胞骨架

靶点

货号

产品名称

抗体分类

种属反应性

应用验证

β-Actin

Ab091004

beta Actin Antibody

Polyclonal

Human, Mouse, Rat

WB, Flow, IHC, IF, ICC

β-Actin

Ab155822

beta Actin Mouse mAb

Monoclonal

Human, Mouse, Rat

WB, Flow, ICC/IF, ELISA

α-Tubulin

Ab088473

Recombinant Alpha Tubulin Antibody

Recombinant

Hamster, Human, Mouse, Rat

WB, IHC, ICC, IF

β-Tubulin

Ab091197

Recombinant beta Tubulin Antibody

Recombinant

Human, Mouse, Rat

WB, IHC, ICC, IF

β-Tubulin

Ab155823

beta Tubulin Mouse mAb

Monoclonal

Human, Mouse, Rat

WB, Flow, ICC/IF, ELISA

γ-Tubulin

Ab104713

Recombinant gamma Tubulin Antibody

Recombinant

Hamster, Human, Mouse, Rat

WB, ICC, IF

β III Tubulin

Ab091154

Recombinant beta III Tubulin Antibody

Recombinant

Human, Mouse, Rat

WB, Flow

Cofilin 1

Ab097098

Cofilin 1 Mouse mAb

Monoclonal

Human

WB, IHC

Vinculin

Ab134234

Recombinant Vinculin Antibody

Recombinant

Human, Mouse, Rat

WB, IHC

Vinculin

Ab169192

Vinculin Mouse mAb

Monoclonal

Human, Mouse, Rat

WB, Flow, ICC/IF, ELISA

α-SMA

Ab088446

Recombinant ACTA2 Antibody

Recombinant

Bovine, Dog, Green Monkey, Human, Mouse, Pig, Rat, Zebrafish

WB, IHC, Flow, IF, ICC

α-SMA

Ab088443

alpha-Smooth Muscle Actin Mouse mAb

Monoclonal

Human, Mouse, Rat

WB, IHC


位于细胞核

靶点

货号

产品名称

抗体分类

种属反应性

应用验证

Histone H3

Ab107980

Recombinant Histone H3 Antibody

Recombinant

Bovine, Dog, Green, Monkey, Human, Mouse, Rat, Zebrafish

WB, IHC, Flow, IP

Lamin B1

Ab112658

Lamin B1 Mouse mAb

Monoclonal

Bovine, Canine, Caprine, Guinea pig, Human, Mouse, Ovine, Porcine, Rabbit, Rat

WB, IHC

Lamin B1

Ab112659

Recombinant Lamin B1 Antibody

Recombinant

Human, Mouse, Rat

WB, IHC

PCNA

Ab120681

Recombinant PCNA Antibody

Recombinant

Bovine, Chicken, Dog, Green Monkey, Human, Rat

WB, Flow, IP

PCNA

Ab155826

PCNA Mouse mAb

Monoclonal

Human, Mouse, Rat

WB, Flow, ICC/IF, ELISA

TBP

Ab130268

TBP Mouse mAb

Monoclonal

Caprine, Cavia, Human, Mouse, Ovine, Porcine, Rat

WB


位于细胞膜

靶点

货号

产品名称

抗体分类

种属反应性

应用验证

Na+/K+-

ATPase α1

Ab088306

ATP1A1 Mouse mAb

Monoclonal

Bovine, Canine, Caprine, Equine, Human, Mouse, Ovine, Porcine, Rabbit, Rat

WB, IHC

Na+/K+-

ATPase α1

Ab128631

Recombinant ATP1A1 Antibody

Recombinant

Chinese hamster, Human, Mouse, Rat

WB, IHC, Flow, IP


位于细胞质/全细胞

靶点

货号

产品名称

抗体分类

种属反应性

应用验证

Cyclophilin B

Ab098512

Cyclophilin B Mouse mAb

Monoclonal

Human, Mouse, Rat

WB, IHC

GAPDH

Ab104788

Recombinant GAPDH Antibody

Recombinant

Human, Mouse

WB, IHC, Flow, IF, ICC, IP

GAPDH

Ab155825

GAPDH Mouse mAb

Monoclonal

Human

WB, Flow, ICC/IF, ELISA

HSP70

Ab108897

Hsp70 Mouse mAb

Monoclonal

Human

WB, IHC, ICC, IF

Cyclophilin A

Ab098507

Cyclophilin A Mouse mAb

Monoclonal

Human, Mouse

WB, IHC, ICC, IF

HSP90

Ab108929

Recombinant Hsp90 Antibody

Recombinant

Human, Mouse, Rat

WB, IF, ICC


位于线粒体

靶点

货号

产品名称

抗体分类

种属反应性

应用验证

COX IV

Ab097414

COX IV Mouse mAb

Monoclonal

Human, Mouse, Rat

WB, IHC, ICC, IF, Flow, IP

COX IV

Ab097410

Recombinant COX IV Antibody

Recombinant

Human

WB, IHC, Flow, IF, ICC, IP

HSP60

Ab108868

Hsp60 Mouse mAb

Monoclonal

Human, Mouse, Rat

WB

VDAC1/Porin

Ab133998

VDAC1/Porin Antibody

Polyclonal

Human, Mouse, Rat

WB, IHC


位于血清

靶点

货号

产品名称

抗体分类

种属反应性

应用验证

Transferrin

Ab132212

Transferrin Mouse mAb

Monoclonal

Human

WB, IHC

Albumin

Ab087998

Albumin Mouse mAb

Monoclonal

Human, Monkey

WB, IHC, ICC, IF, Flow, ELISA

Albumin

Ab176126

Albumin Antibody

Polyclonal

Human

WB, CLIA, LF, GICA, EIA


完善的应用验证:



        如需了解更多产品详情,请前往阿拉丁官网查询。

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